Browsing Waterloo Institute for Nanotechnology by Subject "DNA"
Now showing items 21-40 of 62
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DNA stabilized fluorescent metal nanoclusters for biosensor development
(Elsevier, 2014-03-22)Fluorescent silver, gold and copper nanoclusters (NCs) have emerged for biosensor development. Compared to semiconductor quantum dots, there is less concern about the toxicity of metal NCs, which can be more easily conjugated ... -
DNA-Functionalized Gold Nanoparticles in Macromolecularly Crowded Polymer Solutions
(American Chemical Society, 2012-11-15)DNA-functionalized gold nanoparticles (AuNPs) are one of the most commonly used reagents in nanobiotechnology. They are important not only for practical applications in analytical chemistry and drug delivery, but also for ... -
DNA-Functionalized Monolithic Hydrogels and Gold Nanoparticles for Colorimetric DNA Detection
(American Chemical Society, 2010-12-22)Highly sensitive and selective DNA detection plays a central role in many fields of research, and various assay platforms have been developed. Compared to homogeneous DNA detection, surface-immobilized probes allow washing ... -
DNA-Length-Dependent Fluorescence Signaling on Graphene Oxide Surface
(Wiley, 2012-02-10)Fluorescence energy transfer to graphene oxide is studied using covalently linked DNA probes ranging from 4 to 70 base pairs. The characteristic distance and mechanism of energy transfer are reported. -
Effects of Polyethylene Glycol on DNA Adsorption and Hybridization on Gold Nanoparticles and Graphene Oxide
(American Chemical Society, 2012-10-09)Understanding the interface between DNA and nanomaterials is crucial for rational design and optimization of biosensors and drug delivery systems. For detection and delivery into cells, where high concentrations of cellular ... -
Electrostatically Directed Visual Fluorescence Response of DNA-Functionalized Monolithic Hydrogels for Highly Sensitive Hg2+ Detection
(American Chemical Society, 2011-03-23)Hydrogels are cross-linked hydrophilic polymer networks with low optical background and high loading capacity for immobilization of biomolecules. Importantly, the property of hydrogel can be precisely controlled by changing ... -
Exploring the thermal stability of DNA-linked gold nanoparticles in ionic liquids and molecular solvents
(Royal Society of Chemistry, 2012-11-01)While water is the most commonly used solvent for DNA, many co-solvents have been added for various applications. Ionic liquids (ILs) are molten salts at around room temperature. ILs have been tested as a green solvent for ... -
Extraction of DNA staining dyes from DNA using hydrophobic ionic liquids
(Royal Society of Chemistry, 2013-05-18)The partition of oligonucleotides and DNA staining dyes into a few hydrophobic ionic liquids has been studied, where the oligonucleotides remain in the aqueous phase and all the DNA staining dyes are extracted in the ionic ... -
Fast assembly of non-thiolated DNA on gold surface at lower pH
(Elsevier, 2013-12-01)In a typical protocol for attaching DNA to a gold electrode, thiolated DNA is incubated with the electrode at neutral pH overnight. Here we report fast adsorption of non-thiolated DNA oligomers on gold electrodes at acidic ... -
Fast Molecular Beacon Hybridization in Organic Solvents with Improved Target Specificity
(American Chemical Society, 2010-12-02)DNA hybridization is of tremendous importance in biology, bionanotechnology, and biophysics. Molecular beacons are engineered DNA hairpins with a fluorophore and a quencher labeled on each of the two ends. A target DNA can ... -
Fast pH-assisted functionalization of silver nanoparticles with monothiolated DNA
(Royal Society of Chemistry, 2012-10-18)Attaching monothiolated DNA to silver nanoparticles has been achieved at pH 3.0 in 30 minutes and difficulties associated with DNA attachment to AgNPs at neutral pH have been revealed by studying DNA adsorption kinetics. -
Flow Cytometry-Assisted Detection of Adenosine in Serum with an Immobilized Aptamer Sensor
(American Chemical Society, 2010-05-15)Aptamers are single-stranded nucleic acids that can selectively bind to essentially any molecule of choice. Because of their high stability, low cost, ease of modification, and availability through selection, aptamers hold ... -
Fluorescent sensors using DNA-functionalized graphene oxide
(Springer, 2014-07-02)In the past few years, graphene oxide (GO) has emerged as a unique platform for developing DNA-based biosensors, given the DNA adsorption and fluorescence-quenching properties of GO. Adsorbed DNA probes can be desorbed ... -
The group trend of lanthanides binding to DNA and DNAzymes with a complex but symmetric pattern
(Royal Society of Chemistry, 2014-07-18)Using sensitized Tb3+ luminescence spectroscopy as a tool, binding of 14 lanthanides to a lanthanide-dependent DNAzyme is studied, where the binding affinity is symmetric across the series and the tightest binding occurs ... -
Hydrogel porosity controlling DNA-directed immobilization of gold nanoparticles revealed by DNA melting and scanning helium ion microscopy
(Royal Society of Chemistry, 2012-04-07)Immobilization of nanomaterials is important for many applications, including sensor development, biomaterials design and catalysis. DNA-directed immobilization has been widely used because of its high specificity and ... -
Immobilization of DNA on Magnetic Microparticles for Mercury Enrichment and Detection with Flow Cytometry
(Wiley, 2011-04-26)Mercury detection in water has attracted a lot of research interest due to its highly toxic nature and adverse environmental impact. In particular, the recent discovery of specific binding of HgII to thymine-rich (T-rich) ... -
In Vitro Selection of Chromium-Dependent DNAzymes for Sensing Chromium(III) and Chromium(VI)
(Wiley, 2016-07-04)Chromium is a very important analyte for environmental monitoring, and developing biosensors for chromium is a long-standing analytical challenge. In this work, in vitro selection of RNA-cleaving DNAzymes was carried out ... -
Instantaneous and Quantitative Functionalization of Gold Nanoparticles with Thiolated DNA Using a pH-Assisted and Surfactant-Free Route
(American Chemical Society, 2012-05-02)The attachment of thiolated DNA to gold nanoparticles (AuNPs) has enabled many landmark works in nanobiotechnology. This conjugate chemistry is typically performed using a salt-aging protocol where, in the presence of an ... -
Instantaneous Attachment of an Ultrahigh Density of Nonthiolated DNA to Gold Nanoparticles and Its Applications
(American Chemical Society, 2012-12-11)The last 16 years have witnessed the landmark development of polyvalent thiolated DNA-functionalized gold nanoparticles (AuNP's) possessing striking properties within the emerging field of nanobiotechnology. Many novel ... -
Iron oxide nanozyme catalyzed synthesis of fluorescent polydopamine for light-up Zn2+ detection
(Royal Society of Chemistry, 2016-07-29)Fluorescent polydopamine (FPD) is an interesting material with excellent biocompatibility. However, its preparation is currently a lengthy and potentially dangerous process. We herein employ magnetic iron oxide (Fe3O4) ...