dc.contributor.author | Huang, Po-Jung Jimmy | |
dc.contributor.author | Liu, Juewen | |
dc.date.accessioned | 2017-04-28 16:11:57 (GMT) | |
dc.date.available | 2017-04-28 16:11:57 (GMT) | |
dc.date.issued | 2017-01-15 | |
dc.identifier.uri | http://dx.doi.org/10.1021/acs.analchem.5b04904 | |
dc.identifier.uri | http://hdl.handle.net/10012/11791 | |
dc.description | This document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry, © 2016 American Chemical Society after peer review and technical editing by publisher. To access the final edited and published work see Huang, P.-J. J., & Liu, J. (2016). An Ultrasensitive Light-up Cu2+ Biosensor Using a New DNAzyme Cleaving a Phosphorothioate-Modified Substrate. Analytical Chemistry, 88(6), 3341–3347. https://doi.org/10.1021/acs.analchem.5b04904 | en |
dc.description.abstract | Cu2+ is a very important metal ion in biology, environmental science, and industry. Developing biosensors for Cu2+ is a key topic in analytical chemistry. DNAzyme-based sensors are highly attractive:, for their excellent sensitivity, stability, and programmability, In the past decade, a few Cu2+ biosensors were reported using DNAzymes with DNA cleavage or DNA ligation activity. However, they require unstable ascorbate or imidazole activation. So far, no RNA cleaving DNAzymes specific for Cu2+ are known. In this work, a phosphorothioate (PS) RNA-containing library was used for in vitro selection, and a few new Cu2+-specific RNA-cleaving DNAzymes were isolated. Among them, a DNAzyme named PSCu10 was studied further. It has only eight nucleotides in the enzyme loop with a cleavage rate of 0.1 min(-1) in the presence of 1 mu M Cu2+ at pH 6.0 (its optimal pH). Between the two diastereomers of the PS RNA chiral center, the R-p isomer is 37 times more active than the S-p one. Among the other divalent metal ions, only Hg2+ can cleave the substrate due to its extremely high thiophilicity. A. catalytic beacon sensor was designed with a detection limit of 1.6 nM Cu2+ and extremely high selectivity. PSCu10 is specific for Cu2+; and it has no cleavage in the presence of ascorbate, which reduces Cu2+ to. Cu+. | en |
dc.description.sponsorship | Ontario Ministry of Research Innovation; Natural Sciences and Engineering Research Council (NSERC) of Canada (Discovery Grant); Natural Sciences and Engineering Research Council (NSERC) of Canada (Strategic Project Grant) | en |
dc.language.iso | en | en |
dc.publisher | American Chemical Society | en |
dc.subject | In-Vitro Selection | en |
dc.subject | Lanthanide-Dependent DNAzyme | en |
dc.subject | Catalytic Beacon Sensor | en |
dc.subject | Delta Virus Ribozyme | en |
dc.subject | Aqueous-Solution | en |
dc.subject | Living Cells | en |
dc.subject | Metal-Ions | en |
dc.subject | DNA | en |
dc.subject | Cleavage | en |
dc.subject | Site | en |
dc.title | An Ultrasensitive Light-up Cu2+ Biosensor Using a New DNAzyme Cleaving a Phosphorothioate-Modified Substrate | en |
dc.type | Article | en |
dcterms.bibliographicCitation | Huang, P.-J. J., & Liu, J. (2016). An Ultrasensitive Light-up Cu2+ Biosensor Using a New DNAzyme Cleaving a Phosphorothioate-Modified Substrate. Analytical Chemistry, 88(6), 3341–3347. https://doi.org/10.1021/acs.analchem.5b04904 | en |
uws.contributor.affiliation1 | Faculty of Science | en |
uws.contributor.affiliation2 | Chemistry | en |
uws.contributor.affiliation3 | Waterloo Institute for Nanotechnology (WIN) | en |
uws.typeOfResource | Text | en |
uws.peerReviewStatus | Reviewed | en |
uws.scholarLevel | Faculty | en |